PCR Cloning Kits

PCR cloning kits contain reagents and components for inserting PCR-amplified DNA fragments into cloning vectors. Used in molecular biology laboratories, kits may include vectors, enzymes, buffers, primers, control DNA, and competent cells.

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Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

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Specifications

Promoter	T7
Content And Storage	Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt PCR
Bacterial or Yeast Strain	Not Included
Vector	Blunt DNA Cloning Vectors
Product Line	TOPO, Zero Blunt

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1^R E. coli

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency DH5α-T1^R E. coli

Offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

Pricing & Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	DH5α
Vector	Blunt DNA Cloning Vectors
Product Line	One Shot

Invitrogen™ pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector

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Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells

Enables rapid cloning of one or more genes into virtually any protein expression system

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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R E. coli and PureLink™ Kit

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R E. coli

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

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Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

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Invitrogen™ pLenti6/V5 Directional TOPO™ Cloning Kit

Directional TOPO Cloning site for rapid and efficient directional cloning of blunt-end PCR products

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Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1^R Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System